CellChat

Author

Harvard Chan Bioinformatics Core

Published

August 28, 2025

This code is in this revision.

Clustering

[1] "Create a CellChat object from a data matrix"
The cell barcodes in 'meta' is  1 2 3 4 5 6

Set cell identities for the new CellChat object 
The cell groups used for CellChat analysis are  2_OLD, 2_YOUNG, 3_OLD, 3_YOUNG

Error in identifyOverExpressedGenes(cellchat): For a faster implementation of the Wilcoxon Test, please install the presto package
--------------------------------------------
 devtools::install_github('immunogenomics/presto')
--------------------------------------------
 Otherwise, plase set `do.fast = FALSE` for running the standard Wilcoxon Test!

The number of highly variable ligand-receptor pairs used for signaling inference is 0

triMean is used for calculating the average gene expression per cell group.

Error in data.use[RsubunitsV, , drop = FALSE]: subscript out of bounds

Overall Results

Error in names(x) <- value: 'names' attribute [3] must be the same length as the vector [1]

Error: object 'df.net' not found

Top interactions

Error in h(simpleError(msg, call)): error in evaluating the argument 'x' in selecting a method for function 'unique': object 'df.net' not found

Error: object 'top_ints' not found